A Synthetic Nanotube Antibody for Molecular Recognition

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A synthetic polymer-SWNT complex acts as an effective sensor antibody for analyte binding and signal transduction
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Inventors
Professor Michael Strano
Department of Chemical Engineering, MIT
External Link (srg.mit.edu)
Paul Barone
The Center For Biomedical Innovation, MIT
External Link (cbi.mit.edu)
Daniel Heller
Koch Inst Integrative Cancer Research
Jingqing Zhang
Department of Chemical Engineering, MIT
Managed By
Jon Gilbert
MIT Technology Licensing Officer
Patent Protection

Polymer-nanostructure composition for selective molecular recognition

US Patent Pending US 2011-0257033
Publications
Understanding selective molecular recognition in integrated carbon nanotube–polymer sensors by simulating physical analyte binding on carbon nanotube–polymer scaffolds
Soft Matter, 2014,10, 5991-6004

Applications

  • In vivo or in vitro sensors for biological detection; targeted carbon nanotubes for therapeutic applications

Problem Addressed

This method provides a solution to developing "synthetic antibodies" from synthetic polymers adhered to single walled carbon nanotube surfaces.

Technology

Molecular recognition and signal transduction are two central aspects and challenges to the design of sensor platforms. Natural antibodies are often used as vital components of sensors. However, antibodies are expensive, fragile and easily lose biological activity upon external treatment and exhibit batch-dependent variation, limiting their use. Moreover, certain biological molecules of interest don’t have a naturally existing antibody, including toxins, drugs and explosives. This has driven the search for methods to synthesize artificial antibodies from polymeric materials. SWNT photoluminescence (PL) has been used in sensor platforms for detection of DNA, divalent metal cations, genotoxins, reactive oxygen species and pH, among others. Yet, identifying methods to modulate SWNT PL in response to a specific analyte binding event remains a main challenge in sensor fabrication. Here, it is shown that a random polymer, with little or no affinity for an analyte, will adsorb in specific configurations to SWNTs such that the analyte will both bind to the polymer-SWNT complex and induce a measurable PL signal change. The polymer-nanotube complex can in effect act as a novel nanotube "sensor antibody".

Advantages

  • For target protein or protein therapeutic detection there is no need to tag or label the target to enable detection as the SWNT complex binds directly
  • Works for small molecules and molecules with no known binding partner
  • Allows for continuous detection of the target analyte
  • Multivalent interactions between the polymer and the SWNT are possible ensuring higher efficacy
  • "Synthetic antibodies" may reduce development time compared to antibodies
  • Can also be used to deliver therapeutic molecules, ensuring directed drug release
  • Elucidation of in vivo reaction pathway because fluorescence is tissue transparent