A Rapid Multiplexed Paper Diagnostic Platform

Technology #15365

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 FIG. 1A (prior art) illustrates a perspective view of an exemplary conventional diagnostic strip.  FIG. 1B (prior art) illustrates a close-up view of a sample introduced at an input node of the conventional diagnostic strip of FIG. 1A.  FIG. 1C (prior art) illustrates a close-up view of a reagent provided at a microfluidic channel of the conventional diagnostic strip of FIG. 1A. FIG. 2A illustrates an exploded perspective view of an exemplary diagnostic device showing a network of microfluidic channels.  FIG. 2B is a close-up view of an exemplary reagent provided at a channel in the exemplary diagnostic device of FIG. 2A.  FIG. 2C is a close-view of an exemplary reagent provided at an output node in the exemplary diagnostic device of FIG. 2A.  FIG. 2D is a close-up view of an aperture in an exemplary stencil aligned over an output node in the exemplary diagnostic device of FIG. 2A.FIG. 2E illustrates a perspective view of the exemplary diagnostic device of FIG. 2A in an assembled state.
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Inventors
Professor Lee Gehrke
Department of Engineering, MIT
External Link (imes.mit.edu)
Kimberly Hamad-Schifferli
Lincoln Lab, MIT
Nevan Hanumara
Department of Mechanical Engineering, MIT
David Wood
Jose Gomez-Marquez
Irene Bosch
Jacqueline Linnes
Managed By
Tod Woolf
MIT Technology Licensing Officer
Patent Protection

Systems, devices and methods for multiplexed diagnostics

US Patent 9,488,613

Applications

  • Multiplexed detection of pathogens
  • Adherence/compliance monitoring
  • Detection of prognostic biomarkers that reflect disease severity

Problem Addressed

Monitoring environmental pollution, detection of biomarkers in biological samples and tracking food pathogens require simultaneous identification of multiple of analytes. In developing markets, low cost robust assays of adequate sensitivity and specificity are not available.

Technology

This work presents a compact point-of-care sensor platform capable of simultaneous detection of multiple analytes – proteins, nucleic acids, carbohydrates, toxins, drugs, and drug metabolites. The sensor consists of a paperfluidic platform with multiple channels that guide analytes to sensing areas arranged in a matrix format. Via a sandwich assay using capture and detection antibody pairs, the device can detect multiple analytes in a sample and display the results in a mobile phone or machine readable format, which converts the diagnostics results into a digital format with GPS and time/date data.

Advantages

  • Independent detection of up to 8-10 biomarkers from the same sample
  • Faster speed of detection and analysis
  • Lower cost comparing to traditional analytical lab tools (ELISA, PCR)
  • Conversion of the results into digital format using mobile phone detection scheme