High-throughput Screening System for Rapid Test of Biologics in Animals

Technology #17339

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Inventors
Professor Mehmet Fatih Yanik
Department of Electrical Engineering and Computer Science, MIT
External Link (www.rle.mit.edu)
Tsung-Yao Chang
Department of Electrical Engineering and Computer Science, MIT
Peng Shi
Department of Electrical Engineering and Computer Science, MIT
Joseph Steinmeyer
Department of Electrical Engineering and Computer Science, MIT
Peter Eimon
Research Laboratory of Electronics, MIT
Managed By
Tod Woolf
MIT Technology Licensing Officer
Patent Protection

High-Throughput Organ-Targeted Microinjection Technology for Rapid Screening of Biologics on Animals

US Patent Pending US 2016-0051353
Publications
High-throughput Hyperdimensional Vertebrate Phenotyping
Nature Communications, 4 (2013): 1467
Fully Uutomated Cellular-Resolution Vertebrate Screening Platform with Parallel Animal Processing
Lab on a Chip, 12, no. 4 (2012): 711-716
Organ-targeted High-throughput in Vivo Biologics Screen Identifies Materials for RNA Delivery
Integrative Biology , 6 (2014): 926-934

Applications

  • Automated high-throughput screening of biologics, toxins, drug delivery vehicles, or environmental factors in small animals
  • Drug development

Problem Addressed

In vivo animal studies are an essential part of the preclinical phase of the drug discovery process. Currently, there are no means for high-throughput characterization of biologics using in vivo animal models.

Technology

The technology presented here provides a large-scale in vivo assessment platform that controls delivery of a drug to desired organs of a group of animals to enable high-throughput in vivo screening. The platform enables parallel and automated manipulation of a group of zebrafish larvae for drug injection and subsequent screening.

The setup consists of a microfluidic system that first embeds animals in gel droplets, positions them on a screening plate, and later injects a drug at a specific time, location, and depth into a target organ with assistance from a computer vision algorithm.

Advantages

  • High-throughput injection and screening process
  • Precise control over the injection process (position, depth, time)
  • Automated handling of an animal during entire screen
  • Possibility to work with a number of small vertebrates
  • Excellent repeatability