Single Walled Carbon Nanotubes as In vivo Sensors

Technology #16531

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Front and side schematic views of one of the several binding structures of AT15-SWNT.
Categories
Inventors
Professor Michael Strano
Department of Chemical Engineering, MIT
External Link (srg.mit.edu)
Nigel Reuel
Department of Chemical Engineering, MIT
Thomas McNicholas
Department of Chemical Engineering, MIT
Nicole Iverson
Department of Chemical Engineering, MIT
Managed By
Jon Gilbert
MIT Technology Licensing Officer
Patent Protection

Near infrared fluorescent single walled carbon nanotubes as tissue localizable biosensors

US Patent Pending US 2015-0133752
Publications
Single Molecule Detection of Nitric Oxide Enabled by d(AT)15 DNA Adsorbed to Near Infrared Fluorescent Single-Walled Carbon Nanotubes
JACS, 2011, 133 (3), pp 567–581

Applications

  • Detection of analytes in vivo for diagnostic sensing

Problem Addressed

This technology allows for long-time in vivo detection of analytes.

Technology

The current device presents a photoluminescent single-walled carbon nanotube based nanosensor, embedded in a hydrogel, which can be used for detection of analytes. Nitric oxide (NO) presents an interesting case study for such a sensor. NO is an important cellular signaling molecule, critical for maintaining vascular physiology and regulating immune defense. In addition, its paradoxical roles in carcinogenesis remain unclear, with experimental results that suggest both pro- and anticancer effects. It has been established that there are two fundamental factors complicating the biological effects of NO in vivo: its concentration and its location of production. Accurate detection of NO is essential to understanding its diverse biological roles. Difficulties arise mainly due to its rapid diffusivity and high reactivity with endogenous molecules, including oxygen, exposed thiols, other free radicals, and heme proteins. In many cases, both the resultant short lifetime and its cellular reaction by products affect the accuracy of detection. Chemical approaches to the detection of nitric oxide or its reaction products have been a constant focus of research in recent years, most commonly the design and synthesis of organic fluorophores or quantum dots that modulate fluorescence upon exposure to NO. In this technology, the first fluorescence-based SWNT sensing array is made of single-stranded d(AT)15 DNA oligonucleotide-wrapped SWNTs (AT15-SWNT). This 30-base oligomer imparts SWNTs with the capability of directly, and selectively, quantifying NO concentrations. It is found that the AT -SWNT15 complex is unique in its high selectivity toward NO when compared with a library of other DNA sequences and polymers. The ability to detect nitric oxide quantitatively at the single-molecule level may find applications in new cellular assays for the study of nitric oxide carcinogenesis and chemical signaling, as well as medical diagnostics for inflammation.

Advantages

  • Long-time detection
  • In vivo detection
  • Single-molecule level detection
  • New cellular assays for the study of nitric oxide carcinogenesis and chemical signaling
  • Novel tool for medical diagnostics
  • Implantable platform for in vivo sensing