Concepts and Methods for Controlling Chemical Reaction Kinetics and Interaction Time in Large Systems

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(E) Schematic diagram illustrating the process of scalable and uniform tissue-gel formation without perfusion using SWITCH. GA molecules diffuse into an intact tissue without reacting with biomolecules in pH 3 buffer (SWITCH-Off step). When GA is uniformly dispersed throughout the tissue, the sample is moved to pH 7 buffer (SWITCH-On step) to initiate global gelation/fixation and achieve uniform tissue preservation.(E) Fully co-registered image showing an overlay of 9 rounds of immunostaining. A total of 22 rounds of staining of the same tissue was achieved. R#2 (Iba1), R#3 (GFAP), R#4 (calbindin, CB), R#5 (calretinin, CR), R#7 (PV), R#8 (Neuropeptide Y), R#9 (NeuN), R#18 (SMI-32), and R#19 (PV) were used for co-registration and subsequent quantitative analysis (see Figure 3). The boxed regions indicate the ROI’s shown in panels. (F–G) Scale bar, 300 mm. (F) Vasculature labeling from 9 rounds of staining after co-registration. Scale bar, 200 mm. (G) PV cell counts between rounds 7 and 19. After 12 rounds of imaging, 99% of previously detected PV+ cells were again detected and shown to overlay after co-registration of the datasets. (D) High-temperature (80C) clearing of whole rat brain with and without TG. Scale bars, 6 mm.(F) Rapid clearing of various organs at 80C with and without 50 mM SS. Cleared rat spinal cord is not RI-matched. Scale bars, 3 mm
Categories
Inventors
Professor Kwanghun Chung
Department of Chemical Engineering, MIT
External Link (www.chunglab.org)
Evan Murray
Institute For Medical Engineering and Science, MIT
Jae Hun Cho
Department of Chemical Engineering, MIT
Managed By
Jon Gilbert
MIT Technology Licensing Officer
Patent Protection

Concepts and Methods for Controlling Chemical Reaction Kinetics and Interaction Time in Large Systems

PCT Patent Application Filed
Publications
Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems
Cell, Dec 3, 2015, p. 1500-1514

Applications

The ability to finely control kinetics and molecular interactions in large systems can be applied to any sort of experiment where proteome analysis is invaluable. It is especially useful in any experiment where preservation of the structural and antigenic integrity of the tissue is crucial.

Problem

Many proteome analysis technologies, such as array tomography, that are used to label and visualize 3D pieces of tissue do not allow for the joint measurement of many molecular and anatomical traits in the same piece of tissue, or are difficult to implement in most labs. In recent years, tissue-clearing techniques, such as CLARITY, have enabled scientists to pass each piece of tissue through a few rounds of labeling, in order to visualize the interactions between different cell types and molecules in the same sample. However, the tissue loses structural integrity upon repeated rounds of relabeling. A simple tissue-clearing technique that preserves the structural integrity of tissue after many rounds of relabeling will greatly improve upon current 3D tissue imaging techniques. 

Technology

This invention comprises the SWITCH system (System-Wide control of Interaction Time and kinetics of Chemicals), to tightly control a broad range of chemical reactions in tissue processing and proteomic labeling. A SWITCH-On buffer facilitates chemical reactions between exogenous chemicals and endogenous biomolecules, and a SWTICH-Off buffer suppresses the reactions. These buffers can be used easily to turn tissue into a transparent, heat- and chemical-resistant, architecturally preserved, molecularly labeled structure without needing to fix it with traditional methods. SWITCH can be used to control the rate at which probes penetrate through tissue to label the molecules of interest; thus, it can be used to clear very thick tissue, such as an entire rat brain. The SWITCH system has been used to successfully pass postmortem human tissue through a minimum of 22 rounds of SWITCH labeling with more than 100 targets, all visualized at subcellular resolution. In addition, it has also been used successfully label myelinated axons in intact mouse brain hemispheres. Finally, it has been used successfully to label 1-mm-thick mouse brain blocks with antibodies. The fact that this system is inexpensive and experimentally simple allows for its implementation in most labs. 

Advantages

  • Simple, scalable, broadly-applicable tissue proteome labeling system
  • High-dimensional, multi-scale labeling 
  • Subcellular resolution of dyes, antibodies, and myelination
  • Easy implementation in most labs
  • Virtually unlimited rounds of relabeling possible without disrupting the structural integrity of tissue